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Lab Session 2 | Lab Session 3 | Lab Session 4 | Lab Session 5 | Lab Session 6 | Lab Session 7 | Lab Session 8 | Lab Session 9 | Lab Session 11 | Lab Session 13

Lab Session 1 

Lab Session 1: Preparing to pick a colony of BL21 cells. Lab Session 1: Inoculating 5 mL culture with BL21 colony.

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Lab Session 2: Innoculating 500mL of LB media with BL21 cells that were grown overnight.

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Lab Session 3: Pouring a DNA agarose gel. Lab Session 3: Example digestion gel.

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Lab Session 4: Nickel affinity column setup.

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Lab Session 5: Loading the dialysis cassette. Lab Session 5: Dialyzing proteins.

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Lab Session 6: Adding the running gel. Lab Session 6: Adding the stacking gel. Lab Session 6: Adding tank buffer into SDS gel running apparatus. Lab Session 6: Boiling SDS samples.

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Lab Session 7: SDS setup with comb. Lab Session 7: SDS gel apparatus ready to run. Lab Session 7: SDS gel apparatus running. Lab Session 7: Destaining SDS gels on a rocker. Lab Session 7: Sample SDS gel. Lab Session 7: Final SDS gel.

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Lab Session 8: Removing dialyzed protein from dialysis cassette.

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Lab Session 9: Setting up the PCR reaction. Lab Session 9: PCR reactions ready to be run.

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Lab Session 11: Cell pellet just removed from microcentrifuge. Lab Session 11: Resuspended pellet in Buffer P1. Lab Session 11: Lysed cells. Lab Session 11: Lysed cells. Lab Session 11: Setup of QIAprep spin column.Lab Session 11: Setup of QIAprep spin column. Lab Session 11: Washing QIAprep spin column with Buffer PE. Lab Session 11: Pellet of cell parts and protein (plasmid in supernatant). Lab Session 11: Setup of sequencing reaction. Lab Session 11: Cell culture plate of transformation 50ng.

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Lab Session 13: UV/Vis spectrophotometer. Lab Session 13: 100 uL cuvette reaction vessel. Lab Session 13: UV/Vis spectrum of wild type, no inhibition. PEP substrate added at 2 minutes. Lab Session 13: Inhibition assay data. PEP substrate added at 2 minutes. Inhibitor added at 7 minutes.