Syllabus

Course Meeting Times

Lectures: 1 session / week, 3 hours / session

Course Description

The goal of this course is to provide an insightful overview of the fundamentals of downstream processing for biochemical product recovery. Through reading assignments and homework sets, emphasis is given to fundamental modes of recovery and purification that the scientist and engineer are most likely to encounter in the laboratory and in industrial settings. However, guest lectures by distinguished researchers from both academia and industry in the field of biochemical engineering are also used to demonstrate new concepts and emerging technologies that are likely to benefit biochemical product recovery in the future.

Prerequisites

Familiarity with some of the general aspects of process control and development, as well as general knowledge of chemical engineering principles including thermodynamics, chemical kinetics and transport processes, will be necessary for success in this course. Knowledge of mathematics, especially differential equations and basic statistics (i.e. the normal probability distribution) is necessary for dealing with the engineering aspects of the course. It is expected that students will be proficient in the use of Microsoft® Excel for spreadsheet calculations that are necessary for the completion of many homework assignments.

Grading

Grading will be based on five homework assignments, a midterm exam, and a final exam.

Required Readings

Reading selections are from the sources listed below:

Shutte, H., and M. R. Kula. "Cell Disruption and Isolation of Non-Secreted Products." In Biotechnology: A Multi-Volume Comprehensive Treatise. Vol. 3, Bioprocessing. Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 505-526. ISBN: 9783527283132.

Datar, R. V., and C. G. Rosen. "Cell Debris Removal: Centrifugation and Crossflow Filtration." In Biotechnology: A Multi-Volume Comprehensive Treatise. Vol. 3, Bioprocessing. Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 469-503. ISBN: 9783527283132.

Palmer, D. E., and R. V. Dove. "Ion Exchange Recovery of Proteins." In Comprehensive Biotechnology. Vol. 2, The Principles of Biotechnology: Engineering Considerations. Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 481-488. ISBN: 008032510.

Yarmush, M. L., K. Antonsen, and D. M. Yarmush. "Molecular Sieve Chromatography." In Comprehensive Biotechnology. Vol. 2, The Principles of Biotechnology: Engineering Considerations. Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 489-505. ISBN: 008032510.

Skoog, D. A., D. M. West, and F. J. Holler. "An Introduction to Chromatographic Methods." In Fundamentals of Analytical Chemistry. New York, NY: Saunders College Publishing, 1992, pp. 665-693. ISBN: 9780030749223.

Cleland, J. L., and D. I. C. Wang. "In vitro Protein Refolding." In Biotechnology: A Multi-Volume Comprehensive Treatise. Vol. 3, Bioprocessing. Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 527-555. ISBN: 9783527283132.

Datar, R. V., T. Cartwright, and C. G. Rosen. "Process Economics of Animal Cell and Bacterial Fermentations - A Case Study Analysis of Tissue Plasminogen Activator." Bio/Technology 11, no. 3 (1993) 349-357.

Ladisch, M. R. "Filtration." In Bioseparations Engineering: Principles, Practice, and Economics. New York, NY: John Wiley and Sons, 2001, pp. 36-48. ISBN: 9780471244769.

Tutunjian, R. S. "Ultrafiltration Processes in Biotechnology." In Comprehensive Biotechnology. Vol. 2, The Principles of Biotechnology: Engineering Considerations. Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 411-437. ISBN: 008032510.

Shuler, M. L., and F. Kargi. "Separation of Soluble Products." In Bioprocess Engineeing. Englewood Cliffs, NJ: Prentice Hall PTR, 1991, pp. 325-331. ISBN: 9780134782157.

Kelly, B. D., and T. A. Hatton. "Protein Purification by Liquid-Liquid Extraction." In Biotechnology: A Multi-Volume Comprehensive Treatise. Vol. 3, Bioprocessing. Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1993, pp. 593-616. ISBN: 9783527283132.

Petrides, D., C. L. Cooney, and L. B. Evans. "An Introduction to Biochemical Process Design." In Chemical Engineering Problems in Biotechnology. Edited by M. L. Shuler. New York, NY: American Institute of Chemical Engineers, 1989, pp. 351-391. ISBN:9780816904693.

Ernst, S., et al. "Process Simulation for Recombinant Protein Production: Cost Estimation and Sensitivity Analysis for Heparinase I Expressed in Escherichia coli." Biotechnology and Bioengineering 53, no. 6 (1997): 575-582.

Ladisch, M. R., and K. L. Kohlmann. "Recombinant Human Insulin." Biotechnology Progress 8, no. 6 (1992): 469-478.

Petrides, D., E. Sapidou, and J. Calandranis. "Computer-Aided Process Analysis and Economic-Evaluation for Biosynthetic Human Insulin Production - A Case Study." Biotechnology and Bioengineering 48, no. 5 (1995): 529-541.