Lab Safety (PDF)
WEEK # | LAB TOPICS | PROTOCOLS |
---|---|---|
2 | Basic Skills in a Molecular Biology lab (PDF 1) (PDF 2) | (PDF) |
3 | Sequence Analysis and Vector Design (PDF - 1.6 MB) | (PDF) |
4 | PCR and Digestion (PDF) | (PDF) |
5 | DNA Purification and Subcloning | |
7 | Transformation, Colony Pick-up, Miniprep, and Digestion (PDF) | (PDF) |
8 | Basic Cell Culture | (PDF) |
9 | Primary Culture of Neurons from the Brain | Results from Last Session: GFP (JPG 1) (JPG 2), RFP (JPG 1) (JPG 2) |
10 | SDS-Polyacrylamide Gel Electrophoresis and Western Blotting (PDF) | (PDF) |
11 | Western Blotting 2: Detection | (PDF) |
12 | Brain Tissue Processing (PDF) | (PDF) |