Readings

WEEK # TOPICS READINGS
1

Orientation

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No readings
2

History of stem cell biology and axis of research

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Paper 1

Mintz, B., and K. Illmensee. "Normal Genetically Mosaic Mice Produced from Malignant Teratocarcinoma Cells." Proc Natl Acad Sci U S A 72, no. 9 (September 1975): 3585-9.

Paper 2

Martin, G. "Isolation of a Pluripotent Cell Line from Early Mouse Embryos Cultured in Medium Conditioned by Teratocarcinoma Stem Cells." Proc Natl Acad Sci U S A 78, no. 12 (December 1981): 7634-8.

3

What makes a stem cell a stem cell? Part 1 – Transcription Factors

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Paper 1

Chambers, I., D. Colby, M. Robertson, J. Nichols, S. Lee, S. Tweedie, and A. Smith. "Functional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells." Cell 113, no. 5 (May 30, 2003): 643-55.

Paper 2

Wu, Q., X. Chen, J. Zhang, Y. H. Loh, T. Y. Low, W. Zhang, W. Zhang, S. K. Sze, B. Lim, and H. H. Ng. "Sall4 Interacts with Nanog and Co-occupies Nanog Genomic Sites in Embryonic Stem Cells." J Biol Chem 281, no. 34 (August 25, 2006): 24090-4. Epub 2006 July 13.

4

What makes a stem cell a stem cell? Part 2 – Chromatin Structure

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Paper 1

Bernstein, B. E., T. S. Mikkelsen, X. Xie, M. Kamal, D. J. Huebert, J. Cuff, B. Fry, A. Meissner, M. Wernig, K. Plath, R. Jaenisch, A. Wagschal, R. Feil, S. L. Schreiber, and E. S. Lander. "A Bivalent Chromatin Structure Marks Key Developmental Genes in Embryonic Stem Cells." Cell 125, no. 2 (April 21, 2006): 315-26.

Paper 2

Lagger, S., D. Meunier, M. Mikula, R. Brunmeir, M. Schlederer, M. Artaker, O. Pusch, G. Egger, A. Hagelkruys, W. Mikulits, G. Weitzer, E. W. Muellner, M. Susani, L. Kenner, and C. Seiser. "Crucial Function of Histone Deacetylase 1 for Differentiation of Teratomas in Mice and Humans." EMBO J 29, no. 23 (December 1, 2010): 3992-4007. Epub 2010 October 22.

5

New Technologies

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Paper 1

Fazzio, T. G., J. T. Huff, and B. Panning. "An RNAi Screen of Chromatin Proteins Identifies Tip60-p400 as a Regulator of Embryonic Stem Cell Identity." Cell 134, no. 1 (July 11, 2008): 162-74.

Paper 2

Pardo, M., B. Lang, L. Yu, H. Prosser, A. Bradley, M. M. Babu, and J. Choudhary. "An Expanded Oct4 Interaction Network: Implications for Stem Cell Biology, Development, and Disease." Cell Stem Cell 6, no. 4 (April 2, 2010): 382-95.

6

Field Trip

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No readings
7

Cloning of mammalian cells

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Paper 1

Gurdon, J. B. "Adult Frogs Derived From the Nuclei of Single Somatic Cells." Dev Biol 4 (April 1962): 256-73.

Paper 2

Wilmut, I., A. E. Schnieke, J. McWhir, A. J. Kind, and K. H. Campbell. "Viable Offspring Derived From Fetal and Adult Mammalian Cells." Nature 385 no. 6619 (February 27, 1997): 810-13.

8

Reprogramming and Trans-differentiation - Transcription Factors, 2.0

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Paper 1

Takahashi K., and S. Yamanaka. "Induction of Pluripotent Stem Cells From Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors." Cell 126, no. 4 (August 25, 2006): 663-76.

Paper 2

Ieda, M., J.-D. Fu, P. Delgado-Olguin, V. Vedantham, Y. Hayashi, B. G. Bruneau, and D. Srivastava. "Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes by Defined Factors." Cell 142 no. 3 (August 6, 2010): 375-86.

9

Are cancer-initiating cells stem cells?

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Paper 1

O'Brien, C. A., A. Pollett, S. Gallinger, and J. E. Dick. "A Human Colon Cancer Cell Capable of Initiating Tumour Growth in Immunodeficient Mice." Nature 445, no. 7123 (January 4, 2007): 106-10.

Paper 2

Ben-Porath, I., M. W. Thomson, V. J. Carey, R. Ge, G. W. Bell, A. Regev, and R. A. Weinberg. "An Embryonic Stem Cell-like Gene Expression Signature in Poorly Differentiated Aggressive Human Tumors." Nat Genet 40, no. 5 (May 2008): 499-507.

10

Using lessons from stem cells to treat diabetes

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Paper 1

Kroon, E., L. A. Martinson, K. Kadoya, A. G. Bang, O. G. Kelly, S. Eliazer, H. Young, M. Richardson, N. G. Smart, J. Cunningham, A. D. Agulnick, K. A. D'Amour, M. K. Carpenter, and E. E. Baetge. "Pancreatic Endoderm Derived From Human Embryonic Stem Cells Generates Glucose-responsive Insulin-secreting Cells in vivo." Nat Biotechnol 26, no. 4 (April 2008): 443-52. Epub 2008 February 20.

Paper 2

Zhou, Q., J. Brown, A. Kanarek, J. Rajagopal, and D. A. Melton. "In vivo Reprogramming of Adult Pancreatic Exocrine Cells to Beta-Cells." Nature 455, no. 7213 (October 2, 2008): 627-32.

11

Stem cell therapies

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Paper 1

Thomas, E. D., H. L. Lochte, W. C. Lu, and J. W. Ferrebee. "Intravenous Infusion of Bone Marrow in Patients Receiving Radiation and Chemotherapy." N Engl J Med 257, no. 11 (September 12, 1957): 491-6.

Paper 2

Taguchi, A., T. Soma, H. Tanaka, T. Kanda, H. Nishimura, H. Yoshikawa, Y. Tsukamoto, H. Iso, Y. Fujimori, D. M. Stern, H. Naritomi, and T. Matsuyama. "Administration of CD34+ Cells After Stroke Enhances Neurogenesis via Angiogenesis in a Mouse Model." J Clin Invest 114, no. 3 (August 2004): 330-8.

12

The future of regenerative medicine

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Paper 1

Leong, K. G., B. E. Wang, L. Johnson, and W. Q. Gao. "Generation of a Prostate From a Single Adult Stem Cell." Nature 456, no. 7223 (December 11, 2008): 804-8.

Paper 2

Keirstead, H. S., G. Nistor, G. Bernal, M. Totoiu, F. Cloutier, K. Sharp, and O. Steward. "Human Embryonic Stem Cell-derived Oligodendrocyte Progenitor Cell Transplants Remyelinate and Restore Locomotion After Spinal Cord Injury." J of Neuroscience 25, no. 19 (May 11, 2005): 4694-705.

13

Final assignment

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Paper 1

Mayack, S. R., J. L. Shadrach, F. S. Kim, and A J. Wagers. "Systemic Signals Regulate Ageing and Rejuvenation of Blood Stem Cell Niches. Nature 28, no. 463 (January 28, 2010): 495-500.

Paper 2

Pajcini, K. V., S. Y. Corbel, J. Sage, J. H. Pomerantz, and H. M. Blau. "Transient Inactivation of Rb and ARF Yields Regenerative Cells From Postmitotic Mammalian Muscle." Cell Stem Cell 7, no. 2 (August 6, 2010): 198-213.