WEEK # | TOPICS | READINGS |
---|---|---|
1 |
Orientation | No readings |
2 |
History of stem cell biology and axis of research |
Paper 1Mintz, B., and K. Illmensee. "Normal Genetically Mosaic Mice Produced from Malignant Teratocarcinoma Cells." Proc Natl Acad Sci U S A 72, no. 9 (September 1975): 3585-9. Paper 2Martin, G. "Isolation of a Pluripotent Cell Line from Early Mouse Embryos Cultured in Medium Conditioned by Teratocarcinoma Stem Cells." Proc Natl Acad Sci U S A 78, no. 12 (December 1981): 7634-8. |
3 |
What makes a stem cell a stem cell? Part 1 – Transcription Factors |
Paper 1Chambers, I., D. Colby, M. Robertson, J. Nichols, S. Lee, S. Tweedie, and A. Smith. "Functional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells." Cell 113, no. 5 (May 30, 2003): 643-55. Paper 2Wu, Q., X. Chen, J. Zhang, Y. H. Loh, T. Y. Low, W. Zhang, W. Zhang, S. K. Sze, B. Lim, and H. H. Ng. "Sall4 Interacts with Nanog and Co-occupies Nanog Genomic Sites in Embryonic Stem Cells." J Biol Chem 281, no. 34 (August 25, 2006): 24090-4. Epub 2006 July 13. |
4 |
What makes a stem cell a stem cell? Part 2 – Chromatin Structure |
Paper 1Bernstein, B. E., T. S. Mikkelsen, X. Xie, M. Kamal, D. J. Huebert, J. Cuff, B. Fry, A. Meissner, M. Wernig, K. Plath, R. Jaenisch, A. Wagschal, R. Feil, S. L. Schreiber, and E. S. Lander. "A Bivalent Chromatin Structure Marks Key Developmental Genes in Embryonic Stem Cells." Cell 125, no. 2 (April 21, 2006): 315-26. Paper 2Lagger, S., D. Meunier, M. Mikula, R. Brunmeir, M. Schlederer, M. Artaker, O. Pusch, G. Egger, A. Hagelkruys, W. Mikulits, G. Weitzer, E. W. Muellner, M. Susani, L. Kenner, and C. Seiser. "Crucial Function of Histone Deacetylase 1 for Differentiation of Teratomas in Mice and Humans." EMBO J 29, no. 23 (December 1, 2010): 3992-4007. Epub 2010 October 22. |
5 |
New Technologies |
Paper 1Fazzio, T. G., J. T. Huff, and B. Panning. "An RNAi Screen of Chromatin Proteins Identifies Tip60-p400 as a Regulator of Embryonic Stem Cell Identity." Cell 134, no. 1 (July 11, 2008): 162-74. Paper 2Pardo, M., B. Lang, L. Yu, H. Prosser, A. Bradley, M. M. Babu, and J. Choudhary. "An Expanded Oct4 Interaction Network: Implications for Stem Cell Biology, Development, and Disease." Cell Stem Cell 6, no. 4 (April 2, 2010): 382-95. |
6 |
Field Trip | No readings |
7 |
Cloning of mammalian cells |
Paper 1Gurdon, J. B. "Adult Frogs Derived From the Nuclei of Single Somatic Cells." Dev Biol 4 (April 1962): 256-73. Paper 2Wilmut, I., A. E. Schnieke, J. McWhir, A. J. Kind, and K. H. Campbell. "Viable Offspring Derived From Fetal and Adult Mammalian Cells." Nature 385 no. 6619 (February 27, 1997): 810-13. |
8 |
Reprogramming and Trans-differentiation - Transcription Factors, 2.0 |
Paper 1Takahashi K., and S. Yamanaka. "Induction of Pluripotent Stem Cells From Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors." Cell 126, no. 4 (August 25, 2006): 663-76. Paper 2Ieda, M., J.-D. Fu, P. Delgado-Olguin, V. Vedantham, Y. Hayashi, B. G. Bruneau, and D. Srivastava. "Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes by Defined Factors." Cell 142 no. 3 (August 6, 2010): 375-86. |
9 |
Are cancer-initiating cells stem cells? |
Paper 1O'Brien, C. A., A. Pollett, S. Gallinger, and J. E. Dick. "A Human Colon Cancer Cell Capable of Initiating Tumour Growth in Immunodeficient Mice." Nature 445, no. 7123 (January 4, 2007): 106-10. Paper 2Ben-Porath, I., M. W. Thomson, V. J. Carey, R. Ge, G. W. Bell, A. Regev, and R. A. Weinberg. "An Embryonic Stem Cell-like Gene Expression Signature in Poorly Differentiated Aggressive Human Tumors." Nat Genet 40, no. 5 (May 2008): 499-507. |
10 |
Using lessons from stem cells to treat diabetes |
Paper 1Kroon, E., L. A. Martinson, K. Kadoya, A. G. Bang, O. G. Kelly, S. Eliazer, H. Young, M. Richardson, N. G. Smart, J. Cunningham, A. D. Agulnick, K. A. D'Amour, M. K. Carpenter, and E. E. Baetge. "Pancreatic Endoderm Derived From Human Embryonic Stem Cells Generates Glucose-responsive Insulin-secreting Cells in vivo." Nat Biotechnol 26, no. 4 (April 2008): 443-52. Epub 2008 February 20. Paper 2Zhou, Q., J. Brown, A. Kanarek, J. Rajagopal, and D. A. Melton. "In vivo Reprogramming of Adult Pancreatic Exocrine Cells to Beta-Cells." Nature 455, no. 7213 (October 2, 2008): 627-32. |
11 |
Stem cell therapies |
Paper 1Thomas, E. D., H. L. Lochte, W. C. Lu, and J. W. Ferrebee. "Intravenous Infusion of Bone Marrow in Patients Receiving Radiation and Chemotherapy." N Engl J Med 257, no. 11 (September 12, 1957): 491-6. Paper 2Taguchi, A., T. Soma, H. Tanaka, T. Kanda, H. Nishimura, H. Yoshikawa, Y. Tsukamoto, H. Iso, Y. Fujimori, D. M. Stern, H. Naritomi, and T. Matsuyama. "Administration of CD34+ Cells After Stroke Enhances Neurogenesis via Angiogenesis in a Mouse Model." J Clin Invest 114, no. 3 (August 2004): 330-8. |
12 |
The future of regenerative medicine |
Paper 1Leong, K. G., B. E. Wang, L. Johnson, and W. Q. Gao. "Generation of a Prostate From a Single Adult Stem Cell." Nature 456, no. 7223 (December 11, 2008): 804-8. Paper 2Keirstead, H. S., G. Nistor, G. Bernal, M. Totoiu, F. Cloutier, K. Sharp, and O. Steward. "Human Embryonic Stem Cell-derived Oligodendrocyte Progenitor Cell Transplants Remyelinate and Restore Locomotion After Spinal Cord Injury." J of Neuroscience 25, no. 19 (May 11, 2005): 4694-705. |
13 |
Final assignment |
Paper 1Mayack, S. R., J. L. Shadrach, F. S. Kim, and A J. Wagers. "Systemic Signals Regulate Ageing and Rejuvenation of Blood Stem Cell Niches. Nature 28, no. 463 (January 28, 2010): 495-500. Paper 2Pajcini, K. V., S. Y. Corbel, J. Sage, J. H. Pomerantz, and H. M. Blau. "Transient Inactivation of Rb and ARF Yields Regenerative Cells From Postmitotic Mammalian Muscle." Cell Stem Cell 7, no. 2 (August 6, 2010): 198-213. |