WEEK # | TOPICS | READINGS |
---|---|---|
1 |
Introduction |
[No Readings] |
2 |
The general stress response and sporulation |
Paper 1Schultz, J. E., G. I. Latter, and A. Matin. "Differential Regulation by Cyclic AMP of Starvation Protein Synthesis in Escherichia coli." J Bacteriol 170 (1988): 3903-9. Paper 2Eichenberger, P., M. Fujita, S. T. Jensen, E. M. Conlon, D. Z. Rudner, S. T. Wang, C. Ferguson, K. Haga, T. Sato, J. S. Liu, and R. Losick. "The Program of Gene Transcription for a Single Differentiating Cell Type During Sporulation in Bacillus subtilis." PLoS Biol 2 (2004): 328. |
3 |
Evolution of the general stress response and sporulation |
Paper 1Zambrano, M. M., D. A. Siegele, M. Almirón, A. Tormo, and R. Kolter. "Microbial Competition: Escherichia Coli Mutants that Take Over Stationary Phase Cultures." Science 259 (1993): 1757-60. Paper 2Veening, J. W., E. J. Stewart, T. W. Berngruber, F. Taddei, O. P. Kuipers, and L. W. Hamoen. "Bet-Hedging and Epigenetic Inheritance in Bacterial Cell Development." Proc Natl Acad Sci USA 105 (2008): 4393-8. |
4 |
Protein-protein interactions: Two-component systems |
Paper 1Bray, D., M. D. Levin, and C. J. Morton-Firth. "Receptor Clustering as a Cellular Mechanism to Control Sensitivity." Nature 393 (1998): 85-8. Paper 2Skerker, J. M., B. S. Perchuk, A. Siryaporn, E. A. Lubin, O. Ashenberg, M. Goulian, and M. T. Laub. "Rewiring the Specificity of Two-component Signal Transduction Systems." Cell 133 (2008): 1043-54. |
5 |
sRNAs: Regulating translation mRNA of RpoS (the general stress response activator) |
Paper1Sledjeski, D. D., A. Gupta, and S. Gottesman. "The Small RNA, DsrA, is Essential for the Low Temperature Expression of RpoS During Exponential Growth in Escherichia Coli." EMBO J 15 (1996): 3993-4000. Paper 2Repoila, F., and S. Gottesman. "Signal Transduction Cascade for Regulation of RpoS: Temperature Regulation of DsrA." J Bacteriol 183 (July 2001): 4012-23. |
6 |
Small molecules: The stringent response |
Paper 1Haseltine, W. A., and R. Block. "Synthesis of Guanosine Tetra and Pentaphosphate Requires the Presence of a Codon-Specific Uncharged Transfer Ribonucleic Acid in the Acceptor Site of Ribosomes." Proc Natl Acad Sci USA 70 (1973): 1564–8. Paper 2Kvint, K., A. Farewell, and T. Nyström. "RpoS-Dependent Promoters Require Guanosine Tetraphosphate for Induction Even in the Presence of High Levels of Sigma(s)." J Biol Chem 275 (2000): 14795-8. |
7 |
Field trip to the Laub laboratory |
[No Readings] |
8 |
Pumping out toxins: Antibiotics |
Paper 1Li, X. Z., D. Ma, D. M. Livermore, and H. Nikaido. "Role of Efflux Pump(s) in Intrinsic Resistance of Pseudomonas Aeruginosa: Active Efflux as a Contributing Factor to Beta-Lactam Resistance." Antimicrob Agents Chemother 38 (August 1994): 1742-52. Paper 2Tikhonova, E. B., and H. I. Zgurskaya. "AcrA, AcrB, and TolC of Escherichia coli Form a Stable Intermembrane Multidrug Efflux Complex." J Biol Chem 279, no. 31 (July 30, 2004): 32116-24. |
9 |
Scavenging toxins: Reactive oxidative species |
Paper 1Carlioz, A., and D. Touati. "Isolation of Superoxide Dismutase Mutants in Escherichia coli: is Superoxide Dismutase Necessary for Aerobic life?" EMBO J 5 (1986): 623-30. Paper 2Greenberg, J. T., P. Monach, J. H. Chou, P. D. Josephy, and B. Demple. "Positive Control of a Global Antioxidant Defense Regulon Activated by Superoxide-generating Agents in Escherichia Coli." Proc Natl Acad Sci U S A 87 (August 1990): 6181-5. |
10 |
Promoting tolerance: Extreme heat and cold |
Paper 1Mogk, A., T. Tomoyasu, P. Goloubinoff, S. Rüdiger, D. Röder, H. Langen, and B. Bukau. "Identification of Thermolabile Escherichia Coli Proteins: Prevention and Reversion of Aggregation by DnaK and ClpB." EMBO J 18 (December 15, 1999): 6934-49. Paper 2Guisbert, E., C. Herman, C. Z. Lu, and C. A. Gross. "A Chaperone Network Controls the Heat Shock Response in E. Coli." Genes Dev 18 (2004): 2812-21. |
11 |
Final oral presentations |
[No Readings] |
12 |
Repair calls: Single-stranded DNA damage |
Paper 1Sancar, A., and W. D. Rupp. "A Novel Repair Enzyme: UVRABC Excision Nuclease of Escherichia Coli Cuts a DNA Strand on Both Sides of the Damaged Region." Cell 33 (May 1983): 249-60. Paper 2Mellon, I., and G. N. Champe. "Products of DNA Mismatch Repair Genes mutS and mutL are Required for Transcription-Coupled Nucleotide-Excision Repair of the Lactose Operon in Escherichia Coli." Proc Natl Acad Sci U S A 93 (February 6, 1996): 1292-7. |