Readings

SES # TOPICS READINGS
1 Introduction  
2 Nuclear transfer, part 1

Wilmut, I., A. E. Schnieke, J. McWhir, A. J. Kind, and K. H. S. Campbell. "Viable Offspring Derived from Fetal and Adult Mammalian Cells." Nature 385 (1997): 810-813.

Munsie, M. J., A. E. Michalska, C. M. O'Brien, A. O. Trounson, M. F. Pera, and P. S. Mountford. "Isolation of Pluripotent Embryonic Stem Cells from Reprogrammed Adult Mouse Somatic Cell Nuclei." Curr Biol 10 (2000): 989-992.
3 Nuclear transfer, part 2

Barberi, T., P. Klivenyi, N. Y. Calingasan, H. Lee, H. Kawamata, K. Loonam, A. L. Perrier, et al. "Neural Subtype Specification of Fertilization and Nuclear Transfer Embryonic Stem Cells and Application in Parkinsonian Mice." Nat Biotechnol 21 (2003): 1200-1207.

Egli, D., J. Rosains, G. Birkhoff, and K. Eggan. "Developmental Reprogramming after Chromosome Transfer into Mitotic Mouse Zygotes." Nature 447 (2007): 679-685.
4 Embryonic stem cells and adult stem cells

Chambers, I., D. Colby, M. Robertson, J. Nichols, S. Lee, S. Tweedie, and A. Smith. "Functional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells." Cell 113 (2003): 643-655.

Wagers, A. J., R. I. Sherwood, J. L. Christensen, and I. L. Weissman. "Little Evidence for Developmental Plasticity of Adult Hematopoietic Stem Cells." Science 297 (2002): 2256-2259.
5 Epigenetics - DNA methylation

Okano, M., D. W. Bell, D. A. Haber, and E. Li. "DNA Methyltransferases Dnmt3a and Dnmt3b are Essential for de novo Methylation and Mammalian Development." Cell 99, no. 3 (October 29, 1999): 247-57.

Oda, M., A. Yamagiwa, S. Yamamoto, T. Nakayama, A. Tsumura, H. Sasaki, K. Nakao, E. Li, and M. Okano. "DNA Methylation Regulates Long-range Gene Silencing of an X-linked Homeobox Gene Cluster in a Lineage-specific Manner." Genes & Dev 20 (2006): 3382-3394.
6 Epigenetics - Histone modifications

Weintraub, H., and M. Groudine. "Chromosomal Subunits in Active Genes have an Altered Conformation." Science 193 (1976): 848-856.

Cao, R., L. Wang, H. Wang, L. Xia, H. Erdjument-Bromage, P. Tempst, R. S. Jones, and Y. Zhang. "Role of Histone H3 Lysine 27 Methylation in Polycomb-group Silencing." Science 298 (2002): 1039-1043.
7 Epigenetics - Reading the epigenome

Weber, M., I. Hellmann, M. B. Stadler, L. Ramos, S. Paabo, M. Rebhan, and D. Schubeler. "Distribution, Silencing Potential and Evolutionary Impact of Promoter DNA Methylation in the Human Genome." Nat Genet 39 (2007): 457-466.

Mikkelsen, T. S., M. Ku, D. B. Jaffe, B. Issac, E. Lieberman, G. Giannoukos, P. Alvarez, W. Brockman, T. K. Kim, R. P. Koche, W. Lee, E. Mendenhall, A. O'Donovan, A. Presser, C. Russ, X. Xie, A. Meissner, M. Wernig, R. Jaenisch, C. Nusbaum, E. S. Lander, and B. E. Bernstein. "Genome-wide Maps of Chromatin State in Pluripotent and Lineage-committed Cells." Nature (2007): 553-560.
8 Field trip and written assignment  
9 Oral presentations  
10 Reprogramming by defined factors, part 1

Davis, R. L., H. Weintraub, and A. B. Lassar. "Expression of a Single Transfected cDNA Converts Fibroblasts to Myoblasts." Cell 51 (1987): 987-1000.

Xie, H., M. Ye, R. Feng, and T. Graf. "Stepwise Reprogramming of B Cells into Macrophages." Cell 117 (2004): 663-676.
11 Reprogramming by defined factors, part 2

Takahashi, K., and S. Yamanaka. "Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors." Cell 126 (2006): 663-676.

Maherali, N., R. Sridharan, W. Xie, J. Utikal, S. Eminli, K. Arnold, M. Stadtfeld, R. Yachechko, J. Tchieu, R. Jaenisch, K. Plath, and K. Hochedlinger. "Directly Reprogrammed Fibroblasts Show Global Epigenetic Remodeling and Widespread Tissue Contribution." Cell Stem Cell 1 (2007): 55-70.
12 Ethics discussion Domestic Policy Council. The White House. "Advancing Stem Cell Science Without Destroying Human Life." January 2007. (PDF)