Readings

SES # TOPICS READINGS
1 General Introduction
2 Discovery of Green Fluorescent Protein

Morise, H., O. Shimomura, F. H. Johnson, and J. Winant. "Intermolecular Energy Transfer in the Bioluminescent System of Aequorea." Biochemistry 13, no. 12 (June 4, 1974): 2656-62.

Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. "Green Fluorescent Protein as a Marker for Gene Expression." Science 263, no. 5148 (February 11, 1994): 802-5.
3 Fluorescent Protein Engineering

Yang, F., L. G. Moss, and G. N. Phillips, Jr. "The Molecular Structure of Green Fluorescent Protein." Nat Biotechnol 14, no. 10 (October 1996): 1246-51.

Campbell, R. E., O. Tour, A. E. Palmer, P. A. Steinbach, G. S. Baird, D. A. Zacharias, and R. Y. Tsien. "A Monomeric Red Fluorescent Protein." Proc Natl Acad Sci U.S.A. 99, no. 12 (June 11, 2002): 7877-82.
4 Photoconversion of Fluorescent Proteins Variants to Probe Cellular Dynamics

Terskikh, A., A. Fradkov, G. Ermakova, A. Zaraisky, P. Tan, A. V. Kajava, X. Zhao, S. Lukyanov, M. Matz, S. Kim, I. Weissman, and P. Siebert. "Fluorescent Timer: Protein that Changes Color with Time." Science 290, no. 5496 (November 24, 2000): 1585-8.

Patterson, G. H., and J. Lippincott-Schwartz. "A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells." Science 297, no. 5588 (September 13, 2002): 1873-7.
5 Labeling Probes Other than Fluorescent Proteins

Griffin, B. A., S. R. Adams, and R. Y. Tsien. "Specific Covalent Labeling of Recombinant Protein Molecules Inside live Cells." Science 281, no. 5374 (July 10, 1998): 269-72.

Juillerat, A., C. Heinis, I. Sielaff, J. Barnikow, H. Jaccard, B. Kunz, A. Terskikh, and K. Johnsson. "Engineering Substrate Specificity of O6-Alkylguanine-DNA Alkyltransferase for Specific Protein Labeling in Living Cells." Chembiochem 6, no. 7 (July 2005): 1263-9.
6 Visit to a Fluorescence Microscopy Facility
7 Visualizing the Central Dogma of Molecular Biology

Janicki, S. M., T. Tsukamoto, S. E. Salghetti, W. P. Tansey, R. Sachidanandam, K. V. Prasanth, T. Ried, Y. Shav-Tal, E. Bertrand, R. H. Singer, and D. L. Spector. "From Silencing to Gene Expression: Real-Time Analysis in Single Cells." Cell 116, no. 5 (March 5, 2004): 683-98.

Bratu, D. P., B. J. Cha, M. M. Mhlanga, F. R. Kramer, and S. Tyagi. "Visualizing the Distribution and Transport of mRNAs in Living Cells." U.S.A. Proc Natl Acad Sci 100, no. 23 (November 11, 2003): 13308-13.
8 Fluorescent Sensors of Cell Signaling: FRET

Miyawaki, A., J. Llopis, R. Heim, J. M. McCaffery, J. A. Adams, M. Ikura, and R. Y. Tsien. "Fluorescent Indicators for Ca2+ based on Green Fluorescent Proteins and Calmodulin." Nature 388, no. 6645 (August 28, 1997): 882-7.

Reiff, D. F., A. Ihring, G. Guerrero, E. Y. Isacoff, M. Joesch, J. Nakai, and A. Borst. "In Vivo Performance of Genetically Encoded Indicators of Neural Activity in Flies." J Neurosci 25, no. 19 (May 11, 2005): 4766-78.
9 Quantitative and Ultra-Sensitive Fluorescent Imaging

Phair, R. D., and T. Misteli. "High Mobility of Proteins in the Mammalian Cell Nucleus." Nature 404, no. 6778 (April 6, 2000): 604-9.

Tardin, C., L. Cognet, C. Bats, B. Lounis, and D. Choquet. "Direct Imaging of Lateral Movements of AMPA Receptors inside Synapses." EMBO J 22, no. 18 (September 15, 2003): 4656-65.
10 Student Presentations
11 Power of Fluorescent Imaging for High-throughput Analysis

Wheeler, D. B., S. N. Bailey, D. A. Guertin, A. E. Carpenter, C. O. Higgins, and D. M. Sabatini. "RNAi Living-Cell Microarrays for Loss-Of-Function Screens in Drosophila Melanogaster Cells." Nat Methods 1, no. 2 (November 2004): 127-32. Epub (October 21, 2004).

Neumann, B., M. Held, U. Liebel, H. Erfle, P. Rogers, R. Pepperkok, and J. Ellenberg. "High-Throughput RNAi Screening by Time-Lapse Imaging of Live Human Cells." Nat Methods 3, no. 5 (May 2006): 385-90.
12 Fluorescent Imaging in Living Organisms

Miller, M. J., S.H. Wei, I. Parker, and M. D. Cahalan. "Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node." Science 296, no. 5574 (June 7, 2002): 1869-73.

Amino, R., S. Thiberge, B. Martin, S. Celli, S. Shorte, F. Frischknecht, and R. Menard. "Quantitative Imaging of Plasmodium Transmission from Mosquito to Mammal." Nat Med 12, no. 2 (February 2006): 220-4.