Chemistry (Chemistry Laboratory Techniques) Labs from 5.301
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The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.
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Goal: To manipulate and purify a known amount of a contaminated sample and to record its 1H NMR spectrum, all with minimal loss of material.
Instructor: Dr. Sarah Tabacco
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Goal: You will be given a small sample of cobaltocene (dicyclopentadienylcobalt) in a nitrogen-filled Schlenk flask. Your task is to carry this material through an "obstacle course" of manipulations without allowing your sample to decompose - as determined by UV-Vis spectroscopy. Beware: This sensitive compound will quickly decompose upon exposure to air!
Instructor: Dr. Sarah Tabacco
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Goal: You will be given 2.00 grams of impure naphthalene (mothballs!). Your job is to purify the naphthalene by recrystallization without losing a significant amount of your sample!
Instructor: Dr. Sarah Tabacco
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Goal: In this exercise, you will experiment with the art of growing single crystals. You will need to synthesize Cr(acac)3, then perform several slow recrystallizations to obtain a single crystal of satisfactory quality.
Instructor: Dr. Sarah Tabacco
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Goal: To purify a mixture of two liquids using distillation.
Instructor: Dr. Sarah Tabacco
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Goal: To purify a mixture of two liquids by reduced pressure distillation.
Instructor: Dr. Sarah Tabacco
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Goal: Purify a contaminated compound using silica gel flash column chromatography.
Instructor: Dr. Sarah Tabacco
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Goal: Separate a complex mixture of three compounds using gradient elution flashcolumn chromatography.
Instructor: Dr. Sarah Tabacco
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Goal: You will be given a sample of a solution of bovine heart cytochrome c. You will use the Coomassie® Plus Protein Assay from Pierce to determine the concentration of protein in the sample.
Instructor: Dr. Sarah Tabacco
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Goal: From the CC-level experiment, you know the concentration of protein in your sample. Now you will determine the concentration of iron in bovine heart cytochrome c.
Instructor: Dr. Sarah Tabacco
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